Endosomal trafficking within a single cell consists of various seemingly random processes. These processes include bursts of active transport, endosomal fusion, fission, tubulation, conversions (where one protein is lost from the endosomal membrane, and another is acquired). The endosomal motilities are about 1 micrometer/s and a lot of these events are interrelated and happen simultaneously. Capturing the dynamic behaviour of endosomes to understand biological mechanisms is a challenge. Capitalising on the rapid volumetric imaging capabilities of lattice light sheet based imaging, we have discovered the following novel endosomal behaviours - whole cell level desorption of APPL1 to mediate EGFR trafficking and endosomal collisions resulting in conversions. These examples signify how fast live cell imaging unearths previously undetectable events.