Developing reliable noninvasive methods to assess oocyte and embryo quality has been a critical goal for assisted reproductive technologies in in vitro fertilization (IVF). The low success rate of clinical pregnancy is consistent with the fact that normal transferred embryos fail to implant because of underlying metabolic defects. Changes in metabolic activity could lead to cell death or abnormal embryo development which could be potentially predicted by incorporating noninvasive measurements of metabolism. Metabolic imaging is a well-tested method for detecting metabolic changes in cells and tissues, however, scientific evidence for its use in oocyte and embryo assessments is limited. Measurements of nicotinamide adenine dinucleotide (NADH) could be a useful tool as the auto-fluorescence of NADH is a straightforward representation of mitochondrial function and cellular redox status. The aim of this study was to measure the NADH levels in oocytes and early embryos in development from female mice of different age through live imaging. Oocytes from two groups of mice (Young: 5-8 weeks, Old: 42-45 weeks) were inseminated with control spermatozoa. Then, NADH levels were measured every 2hrs post in vitro fertilisation using confocal microcopy. Fertilisation and embryo development was also assessed. Metaphase II oocytes from young and old females showed significantly different NADH levels of auto-fluorescence (Young: 9469AU, Old: 8793AU, AU: arbitrary units of auto-fluorescence; p<0.05) 2hrs post-fertilisation. Interestingly, there was an increased NADH activity in presumed zygotes from young females that was not observed in old zygotes 4hrs post-fertilisation (Young: 10144AU, Old: 8587AU, p<0.05) as well as 6hrs post-fertilisation (Young: 9679AU, Old: 8546AU, p<0.05). Our study indicates different levels of NADH during early embryo development associated to ageing and metabolic function, demonstrating that noninvasive measurements of NADH could be applied to determine embryo metabolic activity.