Cryo-transmission electron tomography (cryoET) in association with cryo-focused ion beam (cryoFIB) milling enables structural biology studies to be performed directly within the cellular environment. Cryo-preserved cells are milled and a lamella with a thickness of 200-300 nm provides an electron transparent window suitable for cryoET imaging. CryoFIB milling is an effective method, but it is a tedious and time-consuming process, which typically results in ~10 lamellae per day. Here, we introduce an automated method to reproducibly prepare cryo-lamellae on a grid and reduce the amount of human supervision throughout the process. We tested the routine on cryo-preserved Saccharomyces cerevisiae and demonstrate that it is possible to increase the throughput and achieve a rate of 5 lamellae/hour without the need to supervise the preparation. We demonstrate that the quality of the lamellae is consistent throughout the preparation and their compatibility with cryoET analyses.