This work presents advances in high-resolution chemical imaging capability at Australian Synchrotron Infrared (IR) beamline, achieved through the use of an in-house developed synchrotron macro ATR-FTIR microspectroscopic devices [1,2]. Two models of our in-house developed synchrotron macro ATR-FTIR devices, so-called “hybrid” and “soft-contact piezo-controlled” macro ATR-FTIR, will be presented. The "hybrid" macro ATR-FTIR device was developed by modifying the cantilever arm of a standard macro-ATR unit to accept germanium (Ge) ATR elements with different contact facet sizes (i.e. 1 mm, 250 µm and 100 µm in diameter). Coupling synchrotron-IR beam to the Ge ATR element (nGe=4) used in this device, has the effect of not only reducing the beam focus size (improving the lateral resolution) by a factor of 4, but also reducing the mapping step size by 4 times relative to the stage step motion. As a result, the macro ATR-FTIR measurement at Australian Synchrotron IR Beamline can be performed at minimum beam size of 1.9 μm using a 20x objective, and at minimum mapping step size of 250 nm, allowing high-resolution chemical imaging analysis. The device can also be coupled to a temperature control unit to allow temperature-dependent study, as well as measurements that require a fixed temperature such as analysis of dairy products at 4 oC similar to the usual storage condition in a household fridge. Successful applications will be presented across a diverse range of research disciplinary from material & interfacial science, biomedical science, zoology and entomology, to food science and single fibre analysis.